Ph.D., Biological Sciences, Northwestern University
M.S., Biological Systems Engineering, University of Nebraska-Lincoln
B.S., Biological Systems Engineering, University of Nebraska-Lincoln
Nonviral Gene Delivery
Stem Cell Differentiation
Pannier AK, Schubert M, Hofmann T, Kasputis TJ, Herzinger CM, Woollam JA. Method to
obtain micrographs of transparent or semi-transparent specimens using anisotropy contrast.
U.S. Patent No. 10,026,167. July 17, 2018.
Segura T, Shea LD, Pannier AK, Bengali Z, Jang JH, Chung P, and Anderson B.
Controlled surface-associated delivery of genes and oligonucleotides. U.S. Patent No.
7,029,697. April 18, 2006.
Honors and Awards
Big Ten Academic Alliance Academic Leadership Program Fellow (2019-2020)
UNL College of Engineering Faculty Research and Creative Activity Award (2020)
UNL Gamma Sigma Delta Outstanding Researcher Award (2020)
UNL College of Engineering Holling Family Master Teacher Award (2019)
Presidential Early Career Award for Scientist and Engineers (PECASE, awarded in 2019 for
UNL Parents Association Teaching Award (2018)
National Institutes of Health Director’s New Innovator Award (2017)
UNL Outstanding Undergraduate Research Mentor Award (2017)
UNL College of Engineering Tau Beta Pi Distinguished Teaching Award (2017)
UNL Darrell W. Nelson Excellence in Graduate Student Advising Award (2016)
Kozisek T, Hamann A, Nguyen A, Miller M, Plautz S, Pannier AK. 2020. High-Throughput
Screening of Clinically Approved Drugs that Primer Nonviral Gene Delivery to Human Mesenchymal
Stem Cells (Epub ahead of print).
Hamann A, Thomas AK, Kozisek T, Farris E, Lück S, Zhang Y, Pannier AK. 2020.
Screening a chemically defined extracellular matrix mimetic substrate library to identify
substrates that enhance substrate-mediated transfection. Experimental Biology and
Medicine 245(7): 606-619.
Walsh SC, Miles JR, Yao L, Broeckling CD, Rempel LA, Wright-Johnson EC, Pannier AK.
2020. Metabolic compounds within the porcine uterine environment are unique to type of
conceptus present during the early stages of blastocyst elongation. Molecular Reproduction
and Development 87(1):174-190.
Hamann A, Nguyen A, Pannier AK. 2019. Nucleic acid delivery to mesenchymal stem
cells: a review of nonviral delivery methods and applications. Journal of Biological
Mantz A, Rosenthal A, Farris E, Kozisek T, Bittrich E, Nazari S, Schubert E, Schubert M,
Stamm M, Uhlmann P, Pannier AK. 2019. Free polyethylenimine enhances substrate-mediated
gene delivery on titanium substrates modified with RGD-functionalized poly(acrylic acid)
brushes. Frontiers in Chemistry: Poly. Chemistry. 7:51.
Mantz A and Pannier AK. 2019. Biomaterial substrate modifications that influence
cell-material interactions to prime cellular responses to nonviral gene delivery.
Experimental Biology and Medicine. 244(2):100-113.
Hamann A, Broad K, Nguyen A, Pannier AK. 2019. Mechanisms of unprimed and
dexamethasone-primed nonviral gene delivery to human mesenchymal stem cells. Biotechnology
and Bioengineering. 116(2):427-443.
Farris E, Heck K, Lampe AT, Brown DM, Ramer-Tait A, and Pannier AK. 2018. Oral
non-viralgene delivery for applications in DNA vaccination and gene therapy. Current Opinion
in Biomedical Engineering. 7:51-57
Erickson AG, Laughlin TD, Romereim SM, Sargus-Patino CN, Pannier AK, Dudley AT.
2018. A tunable, three-dimensional in vitro culture model of growth plate cartilage using
alginate hydrogel scaffolds. Tissue Engineering, Part A. 24(1-2): 94-105.
Rosenthal A, Mantz A, Bittrich E, Schubert E, Schubert M, Stamm M, Pannier AK,
Uhlmann P. 2018. Biofunctionalization of Titanium Substrates Using Nanoscale Polymer Brushes
with Cell Adhesion Peptides. J Physical Chemistry: B. 122 (25): 6543-6550.